KMID : 0379119940220020117
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Korean Journal of Mycology 1994 Volume.22 No. 2 p.117 ~ p.121
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Variations in Mitochondrial DNA of Pleurotus sajor - caju
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±è°æ¼ö/Kim, Kyung Soo
Â÷µ¿¿/À¯Ã¢Çö/º¯¸í¿Á/Cha, Dong Yeul/You, Chang Hyun/Byun, Myung Ok
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Abstract
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Five strains of Pleurotus sajor-caju were collected from some countries including India and Papua New Guinea. Four strains produced brown fruitbody but the other strain from Papua New Guinea produced white fruitbody. Monokaryons obtained from both strains producing brown fruitbody and white fruitbody were mated each other, They showed different mating types such as brown strain of A©ûA©üB©ûB©ü and white strain of A©ýA©þB©ýB©þ. DNAs were isolated from mycelia of five strains of P. sajor-caju. Mitochondrial DNA was seperated from nuclear DNA by bisbenzimide-CsCl ultracentrifugation. Digestion of the fungal mitochondrial DNA with Eco RI restriction endonuclease yielded from ten to fourteen fragments. Two strains of the five strains showed different restriction pattern of mitochondrial DNA from the other three strains. Summation of the fragment sizes gave a mitochondrial DNA size of about 60 to 65kb.
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